Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF44), Alexa Fluor 488
CD11b 단백질을 인식하는 마우스 모노클로날 항체로, Alexa Fluor 488 형광 표지. 인간 및 비인간 영장류 반응성. 유세포 분석 및 면역세포 형광염색에 적합. 액상 형태, 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF44), Alexa Fluor 488
Applications and Tested Dilution
- Immunocytochemistry (ICC/IF): Assay-dependent
- Flow Cytometry (Flow): 4 µL per 100 µL whole blood or 10⁶ cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ICRF44 |
| Immunogen | Rheumatoid synovial cells and human monocytes |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS with 0.2% BSA |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2539498 |
Additional Formats
Product Specific Information
This antibody recognizes an extracellular epitope of CD11b (Mac-1 alpha), a 165–170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes, and NK cells.
Target Information
CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex, known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3), serves as a receptor for ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X.
CD11b is expressed on monocytes/macrophages, granulocytes, activated lymphocytes, subsets of NK and dendritic cells, and microglia. It is a key marker for microglia and varies in expression with macrophage maturation and tissue type.
CD11b dysfunction is associated with systemic lupus erythematosus and ITGAM-related susceptibility to autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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