Thermo Fisher Scientific Interferon gamma (IFNG) Recombinant Rabbit Monoclonal Antibody (IFNG, 3996R)

Applications

• Immunohistochemistry (Paraffin) (IHC-P): 1–2 µg/mL

Product Specifications

Product Specific Information

• For Immunohistochemistry (PFA-fixed):
  Incubate antibody for 30 minutes at room temperature.
  Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

Interferon gamma (IFNγ, Type II interferon) is a macrophage activation factor produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, and cytokines.
It is a dimeric glycoprotein (~45 kDa) composed of two 146-amino-acid subunits, functioning as a homodimer.
On SDS-PAGE, IFNγ appears as 25, 20, and minor 15.5 kDa bands due to differential glycosylation.
Human IFNγ shows species specificity and does not cross-react with mouse IFNγ.

Functions include:

• Antiviral and tumor antiproliferative activity
• Induction of MHC class I and II
• Macrophage activation
• Enhancement of immunoglobulin secretion by B cells
• Cytokine regulation and synergistic effects with other cytokines

Activation occurs via IFNγ receptor I/II and the JAK-STAT pathway.
Human IFNγ shares ~40% sequence homology with mouse IFNγ.
Expression is upregulated by IL-2, FGF-basic, and EGF, and downregulated by vitamin D3 or DMN.
Mutations in the IFNγ gene are associated with aplastic anemia.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.