Thermo Fisher Scientific SLC12A1 Polyclonal Antibody
SLC12A1 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal 항체로, Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합합니다. NKCC2 단백질의 30-60 아미노산 영역을 면역원으로 사용하며, 인체 및 설치류 시료에 반응합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:250 |
| Immunocytochemistry (ICC/IF) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
| Immunomicroscopy (IM) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 30–60 on NKCC2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The sodium-potassium-chloride cotransporter isoform 2 (NKCC2) is kidney-specific and localized on the apical membrane of the thick ascending limb of Henle’s loop and the macula densa. It mediates NaCl resorption with a stoichiometry of 1Na:1K:2Cl and is sensitive to loop diuretics such as furosemide and bumetanide. Mutations in this gene can cause Bartter-like syndromes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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