Thermo Fisher Scientific Histone Macro-H2A.1 Polyclonal Antibody
Western blot, IHC, ICC/IF, ELISA에 사용 가능한 Rabbit Polyclonal 항체로 Human, Mouse, Rat 반응성. Recombinant macroH2A1 단백질을 면역원으로 사용하며, 핵 및 염색체 위치에서 검출 가능. 연구용으로만 사용.
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Thermo Fisher Scientific Histone Macro-H2A.1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 123–372 of human macroH2A1 (NP_6132582) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.65 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914631 |
Product Specific Information
Positive test controls include: A-549, HeLa, Rat brain.
Target locations: Chromosome, Nucleus.
Immunogen sequence:
KLEAIITPPP AKKAKSPSQK KPVSKKAGGK KGARKSKKKQ GEVSKAASAD STTEGTPADG FTVLSTKSLF LGQKLNLIHS EISNLAGFEV EAIINPTNAD IDLKDDLGNT LEKKGGKEFV EAVLELRKKN GPLEVAGAAV SAGHGLPAKF VIHCNSPVWG ADKCEELLEK TVKNCLALAD DKKLKSIAFP SIGSGRNGFP KQTAAQLILK AISSYFVSTM SSSIKTVYFV LFDSESIGIY VQEMAKLDAN
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fiber in eukaryotes. Two molecules of each core histone (H2A, H2B, H3, and H4) form an octamer around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes.
The linker histone H1 interacts with linker DNA between nucleosomes and functions in chromatin compaction.
This gene, intronless and part of the histone H4 family, lacks polyA tails and contains a palindromic termination element. It is located in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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