Thermo Fisher Scientific Estrogen receptor alpha Polyclonal Antibody
인간 및 흰쥐에서 반응하는 에스트로겐 수용체 알파 폴리클로날 항체로, WB, IHC, ICC, ELISA 등 다양한 응용 가능. 고순도 Protein A 정제, 액상 형태, -20°C 보관. 연구용으로 에스트로겐 수용체 관련 단백질 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200–1:400 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human Estrogen Receptor alpha (amino acids 241–300) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Estrogen Receptors (ER) belong to the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor that, upon binding to estrogen, undergoes a conformational change enabling dimerization and binding to estrogen response elements (ERE) in DNA. This interaction allows ER to regulate gene transcription positively or negatively through coactivator or corepressor recruitment.
There are two forms of estrogen receptors, alpha and beta, encoded by separate genes (ESR1 and ESR2). Alternative RNA splicing produces at least four alpha isoforms (66, 53, 47, and 35 kDa).
ERs are widely expressed in various tissues and are essential for sexual development and reproductive function. They also play roles in bone and are implicated in diseases such as breast cancer, endometrial cancer, and osteoporosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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