Thermo Fisher Scientific CD47 Monoclonal Antibody (B6H12), eBioscience
CD47 단백질을 인식하는 B6H12 클론의 마우스 단클론 항체. Flow cytometry, Western blot, IHC 등 다양한 응용에 사용 가능. 인간 세포에 반응하며 중화 활성 보고됨. 액상 형태로 제공되며 4°C 보관.
- 카탈로그번호
- 14-0479-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD47 Monoclonal Antibody (B6H12), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | 1 publication |
| Immunohistochemistry (IHC) | - | 3 publications |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | - | 2 publications |
| Flow Cytometry (Flow) | 1 µg/test | 17 publications |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Neutralization (Neu) | - | 2 publications |
| Inhibition Assays (IA) | - | 2 publications |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | B6H12 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_837142 |
Additional Available Formats
- Alexa Fluor 488
- Alexa Fluor 700
- APC
- APC-eFluor 780
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 660-120S
- NovaFluor Red 700
- NovaFluor Red 725
- NovaFluor Red 755
- NovaFluor Yellow 610
- NovaFluor Yellow 660
- PE-Cyanine5
- PE-Cyanine7
- PerCP-eFluor 710
- Custom conjugation available
Product Specific Information
The monoclonal antibody B6H12 reacts with CD47, also known as integrin-associated protein (IAP) and neurophilin.
CD47 is a glycosylated five-transmembrane protein involved in cell adhesion through interactions with SIRP and integrins (CD51/CD61, CD41/CD61).
It mediates bi-directional signaling influencing neural synaptic activity and macrophage phagocytosis, and serves as a receptor for thrombospondin.
CD47 expression is found in most hematopoietic cells (T, B cells, monocytes, platelets, erythrocytes) and also in non-hematopoietic tissues.
This antibody has been reported to have neutralizing activity.
Applications Reported: Flow cytometry, immunoprecipitation, western blot, and immunohistochemistry (formalin-fixed paraffin-embedded sections).
Applications Tested:
- Flow cytometry: ≤1 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
- IHC (FFPE): ≤40 µg/mL using low pH antigen retrieval
Optimal titration is recommended per assay.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD47 (Integrin-Associated Protein, IAP) is a ubiquitously expressed transmembrane glycoprotein that interacts with integrins and regulates multiple signaling pathways, including G protein activation and calcium signaling upon cell adhesion.
It functions as a receptor for the C-terminal domain of thrombospondin, playing roles in membrane transport and signal transduction.
CD47 binds to CD172a (SIRPα) on macrophages, inhibiting phagocytosis of CD47-positive cells.
Additionally, CD47-CD172a interactions influence cell migration, B cell adhesion, T cell activation, chondrocyte mechanotransduction, and neuronal development, being abundant in synapse-rich brain and retinal regions.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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