Thermo Fisher Scientific BHC110/LSD1 Polyclonal Antibody
Rabbit polyclonal antibody targeting LSD1 for WB, IHC, IP, and ChIP-Seq applications. Recognizes region 50–100 of human LSD1. Supplied as unconjugated liquid (1 mg/mL). Stored at 4 °C, shipped on wet ice. Recommended shelf life: 1 year.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:15,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
| ChIP-sequencing (ChIP-Seq) | 10 µL/IP |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 50 and 100 of human FAD-binding protein BRAF35-HDAC complex 110 kDa (lysine-specific histone demethylase 1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4 °C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
LSD1 is a histone demethylase that specifically demethylates Lys‑4 of histone H3, a marker for transcriptional activation, acting as a corepressor. It contains a SWIRM domain, an FAD‑binding motif, and an amine oxidase domain. LSD1 functions within histone deacetylase complexes and silences genes by demethylation. It oxidizes substrates via FAD to generate imines that are hydrolyzed. LSD1 demethylates mono‑ and tri‑methylated Lys‑4 of histone H3, and may also demethylate Lys‑9, affecting androgen receptor target gene expression. Mutations can lead to cleft palate, psychomotor retardation, and distinctive facial features.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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