Thermo Fisher Scientific BHC110/LSD1 Polyclonal Antibody
LSD1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, IP, ChIP-Seq 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 1 mg/mL 농도 액상 형태로 제공됩니다. 인체 LSD1에 반응하며 연구용으로 사용됩니다.
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Thermo Fisher Scientific BHC110/LSD1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:15,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
| ChIP-sequencing (ChIP-Seq) | 10 µL/IP |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 50 and 100 of human FAD-binding protein BRAF35-HDAC complex 110 kDa (lysine-specific histone demethylase 1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/Tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
LSD1 is a histone demethylase that specifically demethylates Lys-4 of histone H3, a marker for epigenetic transcriptional activation, acting as a corepressor. It contains a SWIRM domain, FAD-binding motif, and amine oxidase domain. LSD1 functions as part of histone deacetylase complexes and silences genes via demethylation. It demethylates both mono- and tri-methylated Lys-4 of histone H3 and may also demethylate Lys-9, affecting transcriptional repression and androgen receptor target gene expression. Mutations in this gene can cause cleft palate and psychomotor retardation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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