Thermo Fisher Scientific CHOP Polyclonal Antibody
CHOP 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF, ELISA에 사용 가능. Human, Mouse, Rat 반응성. 고순도 친화 크로마토그래피 정제. PBS/glycerol buffer에 보관, -20°C에서 안정적.
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- PA5120034
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein corresponding to amino acids 1–169 of human DDIT3/CHOP (NP_0040742) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.89 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913606 |
Product Specific Information
- Positive test controls: C2C12
- Subcellular localization: Cytoplasm, Nucleus
Immunogen sequence:
MAAESLPFSF GTLSSWELEA WYEDLQEVLS SDENGGTYVS PPGNEEEESK IFTTLDPASL AWLTEEEPEP AEVTSTSQSP HSPDSSQSSL AQEEEEEDQG RTRKRKQSGH SPARAGKQRM KEKEQENERK VAQLAEENER LKQEIERLTR EVEATRRALI DRMVNLHQA
Target Information
GADD153 is a small nuclear protein capable of dimerizing with transcription factors C/EBP alpha and beta. The resulting complex inhibits the normal binding and function of C/EBP to classical binding sites, while gaining activity toward non-classical stress-related targets. Under normal cellular conditions, expression is minimal but is highly upregulated during cellular or ER stress (e.g., tunicamycin treatment, nutrient starvation, reducing agents affecting ER calcium flux).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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