Thermo Fisher Scientific PMCA4 ATPase Monoclonal Antibody (JA9)
PMCA4 ATPase를 특이적으로 검출하는 mouse monoclonal antibody. Western blot, IHC, ELISA 등 다양한 응용에 사용 가능. Human, bovine, pig, rat 시료에 반응. 1 mg/mL 농도의 액상 형태로 제공되며, -20°C에서 보관.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific PMCA4 ATPase Monoclonal Antibody (JA9)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | 11 publications |
| Immunohistochemistry (IHC) | - | 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | 3 publications |
| Flow Cytometry (Flow) | 2 µg / 10^6 cells | - |
| ELISA | 1:15,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Human, Pig, Rat |
| Published Species | Amphibian, Bovine, Human, Mouse, Pig, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | JA9 |
| Immunogen | Purified human erythrocyte PMCA |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2061596 |
Product Specific Information
MA1-914 specifically detects plasma membrane calcium pump PMCA4 ATPase from human, bovine, porcine, and rat tissues.
This antibody has been validated for use in Western blot, immunohistochemistry, and ELISA.
By Western blot, it detects ~129 kDa and ~133 kDa proteins representing PMCA4b and PMCA4a ATPase, respectively.
The antigen used is purified human erythrocyte PMCA ATPase, and the epitope has been mapped to amino acids 51–75 of PMCA4 ATPase, common to both 4a and 4b isoforms.
Target Information
The plasma membrane calcium pump (PMCA ATPase) transports calcium from the cytosol to the extracellular space.
This enzyme is related to other ATPases such as SERCA and Na⁺/K⁺ pumps.
Four genes encode PMCA ATPase, producing about 20 isoforms through alternative splicing.
mRNA studies show that genes 1 and 4 are expressed in most tissues, while genes 2 and 3 are more tissue-specific.
In the pancreas, beta cells express only the 4b isoform, whereas alpha and gamma cells express both 4a and 4b.
Different splice variants exhibit distinct affinities for calcium and calmodulin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.