Thermo Fisher Scientific PMCA4 ATPase Monoclonal Antibody (JA9)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

-

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500

-

Immunocytochemistry (ICC/IF)

Assay-dependent

View 3 publications 3 publications

Flow Cytometry (Flow)

2 µg / 10^6 cells

-

ELISA (ELISA)

1:15,000

-

Product Specifications

Species Reactivity

Bovine, Human, Pig, Rat

Published species

Amphibian, Bovine, Human, Mouse, Pig, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

JA9

Immunogen

Purified human erythrocyte PMCA. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PMCA4 ATPase,uniProtId:P23634-1,ncbiNodeId:9606,antigenRange:1-1241,antigenLength:1241,antigenImageFileName:MA1-914_PMCA4_ATPase_P23634-1_House_mouse.svg,antigenImageFileNamePDP:MA1-914_PMCA4_ATPase_P23634-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2061596

Product Specific Information

MA1-914 specifically detects plasma membrane calcium pump PMCA4 ATPase from human, bovine, porcine and rat tissues.

MA1-914 has been successfully used in Western blot, immunohistochemistry and ELISA procedures. By Western blot, this antibody detects an ~129 kDa and an ~133 kDa protein representing PMCA4b and PMCA4a ATPase, respectively.

The MA1-914 antigen is purified human erythrocyte PMCA ATPase. The epitope for this antibody has been mapped to amino acids 51-75 of PMCA4 ATPase. This sequence is common to both PMCA4a and 4b ATPase.

Target Information

The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the SERCA ATPase and the sodium/K+ pump. There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin (Cat. # MA3-917 and MA3-918).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.