Thermo Fisher Scientific Periostin Monoclonal Antibody (OTI2B2)
Periostin 단백질을 인식하는 Mouse IgG2a 단클론 항체로, Western blot과 IHC(Paraffin)에서 검증됨. 인간 시료에 반응하며, 액상 형태로 제공. 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2B2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 538–781 of POSTN produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2723456 |
Target Information
Periostin (PN), also known as osteoblast-specific factor 2 (OSF-2), is a disulfide-linked protein originally identified as an osteoblast-specific factor. It is a secreted protein that binds heparin and serves as a ligand for αVβ3 and αVβ5 integrins. In preosteoblasts, Periostin functions as a cell adhesion molecule, contributing to osteoblast recruitment, spreading, and attachment.
Periostin is mainly expressed in the lower gastrointestinal tract, aorta, stomach, placenta, uterus, and breast tissues, but is upregulated in epithelial ovarian tumors and overexpressed in breast cancer. Its expression is increased by bone morphogenetic protein (BMP2) and transforming growth factor beta 1 (TGF-β1). Structurally, Periostin contains a signal sequence, a cysteine-rich domain, four fasciclin-like repeated domains, and a C-terminal domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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