Thermo Fisher Scientific TCF12 Monoclonal Antibody (OTI4D6)
TCF12 단백질을 인식하는 Mouse monoclonal antibody. Western blot 및 IHC(Paraffin) 검증 완료. 고순도 Affinity Chromatography 정제, PBS/BSA/glycerol buffer에 보관. 인체 시료에 반응하며 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4D6 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–250 of TCF12 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2725490 |
Target Information
TCF12, also known as HTF4, is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG.
It is expressed in many tissues, including skeletal muscle, thymus, and B- and T-cells. TCF12 may regulate lineage-specific gene expression through heterodimer formation with other bHLH E-proteins.
Together with E2A, TCF12 is required to block thymocyte proliferation prior to pre-TCR expression and is critical for proper T cell differentiation.
Recent studies indicate that TCF12 is essential for the development of invariant natural killer T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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