Thermo Fisher Scientific Nucleolin Monoclonal Antibody (4E2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunohistochemistry (IHC)

1-10 µg/mL

-

Immunocytochemistry (ICC/IF)

1-10 µg/mL

View 4 publications 4 publications

ChIP assay (ChIP)

Assay-dependent

-

in situ PLA (PLA)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human

Published species

Bovine, Human, Rabbit

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4E2

Immunogen

Human nucleolin protein from Raji cell extract. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Nucleolin,uniProtId:P19338-1,ncbiNodeId:9606,antigenRange:1-710,antigenLength:710,antigenImageFileName:MA1-20800_Nucleolin_P19338-1_House_mouse.svg,antigenImageFileNamePDP:MA1-20800_Nucleolin_P19338-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 50% glycerol

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_560407

Product Specific Information

Recommended positive controls: Raji cell extract.

This antibody does not react with mouse protein.

Target Information

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.