Thermo Fisher Scientific RACGAP1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:500

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 353-632 of human RGAP1 (NP_0011195761) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RACGAP1,uniProtId:Q9H0H5-1,ncbiNodeId:9606,antigenRange:353-632,antigenLength:632,antigenImageFileName:PA5-92378_RACGAP1_Q9H0H5-1_Rabbit.svg,antigenImageFileNamePDP:PA5-92378_RACGAP1_Q9H0H5-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.51 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2806463

Product Specific Information

Immunogen sequence: DFVSQTSPMI PSIVVHCVNE IEQRGLTETG LYRISGCDRT VKELKEKFLR VKTVPLLSKV DDIHAICSLL KDFLRNLKEP LLTFRLNRAF MEAAEITDED NSIAAMYQAV GELPQANRDT LAFLMIHLQR VAQSPHTKMD VANLAKVFGP TIVAHAVPNP DPVTMLQDIK RQPKVVERLL SLPLEYWSQF MMVEQENIDP LHVIENSNAF STPQTPDIKV SLLGPVTTPE HQLLKTPSSS SLSQRVRSTL TKNTPRFGSK SKSATNLGRQ GNFFASPMLK; Positive Samples: HeLa, K-562, Jurkat; Cellular Location: Cell membrane, Cleavage furrow, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Midbody, Nucleus, Peripheral membrane protein, acrosome, cytoskeleton, secretory vesicle, spindle

Target Information

RAC, a small GTPase in the ras family, regulates the formation of membrane ruffles, lamellipodia and filopodia. It is involved in cytoskeletal actin organization and transformation. The p21 activated kinases (PAKs) are direct targets of active Rac and Cdc42 which can induce the assembly of polarized cytoskeletal structures when expressed in fibroblasts. RAC1 and RAC2 are also essential components of NADPH oxidase, the enzyme responsible for generating free radicals.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.