Thermo Fisher Scientific SIRT1 Monoclonal Antibody (19A7AB4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

0.5 µg/mL

-

ELISA (ELISA)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

19A7AB4

Immunogen

Recombinant human SIRT1, 2, 3, 4.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C, do not freeze

RRID

AB_2535904

Product Specific Information

The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Target Information

NAD-dependent protein deacetylase sirtuin-1 (SIRT1) links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, repsonse to DNA damage, metabolism, apoptosis, and autophagy. SIRT1 can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histone and DNA, leading to transcriptional repression. It is essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5`-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.