Thermo Fisher Scientific CYP51A1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 245-420 of CYP51A1 (NP_0007771) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CYP51A1,uniProtId:Q16850-1,ncbiNodeId:9606,antigenRange:245-420,antigenLength:509,antigenImageFileName:PA5-89258_CYP51A1_Q16850-1_Rabbit.svg,antigenImageFileNamePDP:PA5-89258_CYP51A1_Q16850-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.34 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2805449

Product Specific Information

Immunogen sequence: WLLPGWLPLP SFRRRDRAHR EIKDIFYKAI QKRRQSQEKI DDILQTLLDA TYKDGRPLTD DEVAGMLIGL LLAGQHTSST TSAWMGFFLA RDKTLQKKCY LEQKTVCGEN LPPLTYDQLK DLNLLDRCIK ETLRLRPPIM IMMRMARTPQ TVAGYTIPPG HQVCVSPTVN QRLKDS

Positive Samples: Cellular Location: Endoplasmic reticulum membrane, Microsome membrane

Target Information

P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds. Several P450 enzymes have been classified by sequence similarities as members of the CYP1A and CYP2A subfamilies. CYP51A1 (cytochrome P450, family 51, subfamily A, polypeptide 1), also known as LDM (lanosterol 14-alpha demethylase) or cytochrome P450-14DM, is a 503 amino acid protein localized to endoplasmic reticulum membrane. CYP51A1 is an important enzyme for zymosterol and steroid biosynthesis. CYP51A1 catalyzes C14-demethylation of lanosterol, transforming lanosterol into 4,4`-dimethyl cholesta-8,14,24-triene-3-beta-ol. CYP51A1 is ubiquitously expressed, with highest levels found in liver, ovary, testis, lung, kidney and prostate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.