Thermo Fisher Scientific CD80 Monoclonal Antibody (OTI7E4), TrueMAB
인간 CD80 단백질을 인식하는 마우스 단클론 항체로, WB 및 ICC/IF에 적합합니다. TrueMAB 포맷으로 고순도 정제되었으며, 무보존제 PBS 버퍼에 동결건조 형태로 제공됩니다. 연구용으로 T세포 활성화 관련 연구에 활용 가능합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI7E4 |
| Immunogen | Full length human recombinant protein of human CD80 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
CD80 (B7-1) and CD86 (B7-2) are ligands for T cell costimulatory molecule CD28 and inhibitory receptor CTLA-4 (CD152).
Both are expressed on antigen-presenting cells and are essential for T cell activation.
CD80 is rapidly induced on activated B cells, EBV-transformed B cell lines, Burkitt’s lymphoma cells, follicular B lymphoma cells, T cells, and monocytes.
The B-lymphocyte activation antigen B7-1 regulates T lymphocytes via binding to CD28 and CTLA4.
Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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