Thermo Fisher Scientific KRAS Monoclonal Antibody (4E2)
KRAS 단백질을 인식하는 Mouse monoclonal antibody(4E2)로 Western blot 및 ELISA에 적합. Human 시료에 반응하며 PBS buffer에 보관. Affinity chromatography로 정제되어 고순도 확보. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1–5 µg/mL
ELISA
- Tested Dilution: 10 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4E2 |
| Immunogen | KRAS (NP_004976, 16 a.a. approximately 125 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
KSALTIQLIQ NHFVDEYDPT IEDSYRKQVV IDGETCLLDI LDTAGQEEYS AMRDQYMRTG EGFLCVFAIN NTKSFEDIHH YREQIKRVKD SEDVPMVLVG NKCDLPSRTV
Target Information
K-RAS (GTPase KRas) belongs to the Ras oncogene family. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. It plays an important role in the regulation of cell proliferation and has a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes in colorectal cancer cells in a ZNF304-dependent manner.
Mutations in the gene can result in leukemia acute myelogenous, leukemia juvenile myelomonocytic, Noonan Syndrome, gastric cancer, and cardiofaciocutaneous syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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