Thermo Fisher Scientific CaMKII alpha Monoclonal Antibody (6G9), PerCP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:2,000-1:10,000

-

Immunocytochemistry (ICC/IF)

1:50

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Radioimmune Assays (RIA)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

6G9

Immunogen

Partially purified rat CaMKII if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CaMKII alpha,uniProtId:P11275-1,ncbiNodeId:10114,antigenRange:1-478,antigenLength:478,antigenImageFileName:MA5-45145_CaMKII_alpha_P11275-1_House_mouse.svg,antigenImageFileNamePDP:MA5-45145_CaMKII_alpha_P11275-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

PerCP PerCP PerCP

View additional formats

• APC
• FITC
• Request custom conjugation

Excitation/Emission Max

482/675 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.2, with 0.5M EDTA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931599

Product Specific Information

0.1 µg/mL was sufficient for detection of CamKII in 20 µg rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.|Detects approximately 50-60kDa. Recognizes both phosphorylated and non-phosphorylated forms.

Target Information

CAMK2A (CaMKII alpha) is a serine/threonine kinase that regulates a number of cellular functions including neuronal processes, gene expression, carbohydrate metabolism, cytoskeletal function, and calcium homeostatsis. Autophosphorylation of threonine 286 in the presence of Ca2+ and calmodulin activates CAMK2A with the ability to switch from calmodulin-dependent to a calmodulin independent activity. CaMKII is an ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+/calmodulin (CaM) binding and autophosphorylation and has been implicated in regulation of the cell cycle and transcription. It is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca2+ transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.