Thermo Fisher Scientific CaMKII alpha Monoclonal Antibody (6G9), PerCP
CaMKII alpha 단백질을 인식하는 Thermo Fisher Scientific의 6G9 클론 단일클론 항체로 PerCP 형광 표지. WB, IHC, ICC 등 다양한 응용 가능. Bovine, Human, Mouse, Rat 반응성. 고순도 Protein G 정제, 1 mg/mL 농도.
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Thermo Fisher Scientific CaMKII alpha Monoclonal Antibody (6G9), PerCP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:2,000–1:10,000 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
| Radioimmune Assays (RIA) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6G9 |
| Immunogen | Partially purified rat CaMKII |
| Conjugate | PerCP |
| Excitation/Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.2, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931599 |
Additional Formats
Product Specific Information
0.1 µg/mL was sufficient for detection of CaMKII in 20 µg rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary antibody. Detects approximately 50–60 kDa. Recognizes both phosphorylated and non-phosphorylated forms.
Target Information
CAMK2A (CaMKII alpha) is a serine/threonine kinase that regulates various cellular functions including neuronal processes, gene expression, carbohydrate metabolism, cytoskeletal function, and calcium homeostasis. Autophosphorylation of threonine 286 activates CAMK2A, enabling calmodulin-independent activity. CaMKII is widely expressed and plays key roles in dendritic spine and synapse formation, neuronal plasticity, and calcium transport regulation in skeletal muscle. In neurons, it binds and bundles actin filaments, contributing to cytoskeletal reorganization during plasticity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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