Thermo Fisher Scientific Phospho-Cyclin E (Thr77) Recombinant Rabbit Monoclonal Antibody (SD2025)
인간 Cyclin E1 Thr77 인산화 부위를 인식하는 재조합 토끼 단클론 항체로, WB, IHC, ICC/IF에 적합합니다. 높은 특이성과 민감도, 로트 간 일관성, 동물 유래 성분 없는 포뮬레이션을 제공합니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific Phospho-Cyclin E (Thr77) Recombinant Rabbit Monoclonal Antibody (SD2025)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SD2025 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Thr77 of Human Cyclin E1 (aa 51–100) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809639 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
Cyclin E belongs to the highly conserved cyclin family, characterized by periodic changes in protein abundance through the cell cycle. Cyclins regulate CDK kinases and exhibit distinct expression and degradation patterns that coordinate mitotic events.
Cyclin E forms a complex with CDK2, required for G1/S transition. The protein accumulates at the G1-S boundary and is degraded during S phase. Overexpression has been linked to tumorigenesis due to chromosome instability.
Cyclin E also associates with NPAT, involved in histone gene expression and cell-cycle progression in the absence of pRB. Multiple splice variants exist, with some lacking detailed sequence information.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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