Thermo Fisher Scientific UBQLN4/CIP75/Ubiquilin 4 Recombinant Rabbit Monoclonal Antibody (BLR145J)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Flow Cytometry (Flow)

1 µL/10^6 cells

-

Immunoprecipitation (IP)

20 µL/mg of lysate

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293F

Class

Recombinant Monoclonal

Type

Antibody

Clone

BLR145J

Immunogen

Recombinant protein representing Human Ubiquilin-4 (residues 2-601). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Ubiquilin 4,uniProtId:Q9NRR5-1,ncbiNodeId:9606,antigenRange:2-601,antigenLength:601,antigenImageFileName:A700-145_Ubiquilin_4_Q9NRR5-1_Rabbit.svg,antigenImageFileNamePDP:A700-145_Ubiquilin_4_Q9NRR5-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

50 µg/mL

Purification

purified

Storage buffer

BBS, pH 8.2, with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Immunocytochemistry: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Target Information

The ubiquitin-proteosome pathway of protein degradation is one of the mechanisms that ensure the proper level of cellular proteins. The ubiquitin-like (UbL) and ubiquitin-associated (UBA) domain containing protein family is thought to be involved in proteosomal degradation. One such protein is CIP75, also known as ubiquilin-4, interacts with a number of proteins such as Ataxin-1 and Connexin-43, resulting in an increased rate of turnover of these proteins. Overexpression CIP75 led to a significant reduction of Connexin-43 half-life, with the opposite being observed in siRNA knockdown experiments. CIP75 contains an N-terminal UbL domain which is thought to interact with proteins of the 26S proteosome complex and a C-terminal UBA domain which appears to mediate its interaction with Connexin-75. At least three isoforms of CIP75 are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.