Thermo Fisher Scientific Phospho-SHP-1 (Ser591) Polyclonal Antibody

Applications

• Western Blot (WB): 1:1,000 dilution

Product Specifications

Product Specific Information

This antibody was cross-adsorbed to a non-specific phospho-serine peptide and then affinity-purified using phospho-SHP1 (Ser-591) peptide. The antibody detects a 68 kDa band on SDS-PAGE immunoblots of human Jurkat and A431 cells treated with pervanadate, as well as A431 cells treated with EGF.

Target Information

SHP-1 (PTPN6) is a non-receptor protein tyrosine phosphatase expressed primarily in hematopoietic cells. It contains two SH2 domains, a tyrosine phosphatase catalytic domain, and a carboxy-terminal regulatory domain. SHP-1 dephosphorylates target proteins to downregulate several tyrosine kinase-regulated pathways. In hematopoietic cells, the amino-terminal SH2 domain of SHP-1 binds to tyrosine-phosphorylated erythropoietin receptors (EpoR) to negatively regulate hematopoietic growth. Overexpression of SHP-1 in epithelial cells results in dephosphorylation of the Ros receptor tyrosine kinase and subsequent downregulation of Ros-dependent cell proliferation and transformation. Following ligand binding in myeloid cells, SHP-1 associates with the IL-3R beta chain and downregulates IL-3-induced tyrosine phosphorylation and cell proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.