Thermo Fisher Scientific CD200 Monoclonal Antibody (OX104), APC-eFluor 780, eBioscience

Applications and Tested Dilution

Flow Cytometry (Flow)

• Tested Dilution: 5 µL (0.25 µg)/test
• View 2 publications

Product Specifications

Additional Formats

• Unconjugated
• APC
• Biotin
• Brilliant Violet 711
• eFluor 450
• FITC
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 710
• NovaFluor Yellow 610
• NovaFluor Yellow 700
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
The OX104 monoclonal antibody recognizes human CD200 (OX2), a member of the immunoglobulin superfamily with two Ig domains, a transmembrane, and a cytoplasmic domain. CD200 is expressed on resting and activated B cells, subsets of T cells, keratinocytes, peripheral and central nerve cells, follicular dendritic cells, and ovarian cells. Interaction with CD200R modulates macrophage activation, inhibits mast cell degranulation, and reduces TNF-α and IL-13 secretion. CD200 expression on trophoblasts suggests a role in maternal tolerance.

Applications Reported:
Suitable for flow cytometric analysis.

Applications Tested:
Pre-diluted and tested by flow cytometry on normal human peripheral blood cells. Recommended use: 5 µL (0.25 µg) per test (100 µL final volume). Cell count per test: 10⁵–10⁸ cells (empirically determined).

Fluorochrome Information:
APC-eFluor 780 emits at 780 nm and is excited by the red laser (633–647 nm). Ensure instrument compatibility.

Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect from light.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product #00-8222-49)
• 1-step Fix/Lyse Solution (Product #00-5333-57)

Excitation: 633–647 nm
Emission: 780 nm
Laser: Red Laser

Target Information

CD200 is a type I membrane glycoprotein with two immunoglobulin domains, part of the Ig superfamily. It interacts with CD200R on myeloid cells to inhibit macrophage activation. Homologues exist in herpesviruses and poxviruses. CD200 regulates myeloid cell activity and delivers inhibitory signals in diverse tissues. Multiple alternatively spliced variants exist.
Associated diseases include Mantle Cell Lymphoma and Primary Hypertrophic Osteoarthropathy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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