Thermo Fisher Scientific CYP4F3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide directed towards the N-terminal of human CYP4F3 (aa 35-84). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CYP4F3,uniProtId:Q08477-1,ncbiNodeId:9606,antigenRange:35-84,antigenLength:520,antigenImageFileName:PA5-42066_CYP4F3_Q08477-1_Rabbit.svg,antigenImageFileNamePDP:PA5-42066_CYP4F3_Q08477-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2608047

Product Specific Information

Peptide sequence: LAWTYTFYDN CCRLRCFPQP PKRNWFLGHL GLIHSSEEGL LYTQSLACTF

Sequence homology: Dog: 86%; Human: 100%; Mouse: 85%; Rat: 85%

Target Information

Cytochrome P450 super family proteins are monooxygenases which catalyze multiple biochemical reactions involved in drug metabolism and synthesis of cholesterol, steroids and lipids. CYP4F3, a 60 kDa protein, is a novel member of this family. It consists of a conserved heme-binding domain near the C-terminus and catalyzes the u-hydroxylation of LTB4. CYP4F3 is localized in the ER and plays a vital role in the inactivation of LTB4 and controlling inflammation. CYP4F3 exists in two functionally distinct isoforms: CYP4F3A, a neutrophil isoform, is particularly expressed in neutrophils and myeloid cells and catalyzes the microsomal u-hydroxylation of LTB4. It interacts with CD11b and myeloperoxidase on myeloid cells and regulates cellular development in bone marrow. CYP4F3B, a liver isoform, catalyzes the conversion of arachidonic acid to 20-HETE, a potent activator of PKC. Its role has been implicated in cell proliferation, vascular tone and natriuresis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.