
Thermo Fisher Scientific VSV-G Epitope Tag Polyclonal Antibody, DyLight 649
VSV-G epito프 태그를 인식하는 DyLight 649 결합 폴리클로날 항체로, Western blot, ICC/IF, ELISA에 적합. 고순도 친화 크로마토그래피 정제 제품이며, 장기 보관 시 -20°C에서 안정적. 연구용 단백질 발현 검출에 이상적.
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Thermo Fisher Scientific VSV-G Epitope Tag Polyclonal Antibody, DyLight 649
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | >1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to aa 501–511 (Y-T-D-I-E-M-N-R-L-G-K) of vesicular stomatitis virus glycoprotein (VSV-G), conjugated to KLH using maleimide |
| Conjugate | DyLight™ 649 |
| Excitation / Emission Max | 651 / 673 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.02M potassium phosphate (pH 7.2), 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
This affinity-purified antibody is directed against the VSV-G epitope tag and is useful for detecting its presence in overexpressed proteins across various assays.
It recognizes the VSV-G epitope tag (Tyr-Thr-Asp-Ile-Glu-Met-Asn-Arg-Leu-Gly-Lys) fused to either the amino or carboxy termini of targeted proteins in transfected or transformed cells.
Target Information
Vesicular stomatitis virus (VSV) is an enveloped RNA virus from the Rhabdoviridae family.
It is released from host cells by budding, and its glycoprotein (VSV G) contains a membrane-proximal stem domain required for efficient budding.
Sequence: YTDIEMNRLGK.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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