Thermo Fisher Scientific K-Ras Polyclonal Antibody
Thermo Fisher Scientific의 K-Ras Polyclonal Antibody는 인간, 마우스, 랫트에 반응하는 rabbit IgG 기반 항체입니다. Western blot, ICC/IF, ELISA에 적합하며 세포막 및 세포질에서 K-RAS 단백질 검출에 활용됩니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 30–189 of human KRAS (NP_203524.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.98 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804790 |
Product Specific Information
- Positive Samples: A-549, HeLa, Raji, Mouse spleen, Mouse lung, Rat brain
- Cellular Location: Cell membrane, Cytoplasm, Cytoplasmic side, Lipid-anchor, Cytosol
Immunogen Sequence:
DEYDPTIEDS YRKQVVIDGE TCLLDILDTA GQEEYSAMRD QYMRTGEGFL CVFAINNTKS FEDIHHYREQ IKRVKDSEDV PMVLVGNKCD LPSRTVDTKQ AQDLARSYGI PFIETSAKTR QRVEDAFYTL VREIRQYRLK KISKEEKTPG CVKIKKCIIM
Target Information
K-RAS (GTPase KRas) belongs to the Ras oncogene family. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. It plays an important role in regulating cell proliferation and promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes in colorectal cancer cells in a ZNF304-dependent manner.
Mutations in the K-RAS gene can result in leukemia (acute myelogenous, juvenile myelomonocytic), Noonan Syndrome, gastric cancer, and cardiofaciocutaneous syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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