Thermo Fisher Scientific Nestin Polyclonal Antibody
Nestin 단백질을 인식하는 Rabbit Polyclonal 항체로, Human, Mouse, Rat 시료에 반응합니다. Western blot, IHC, ICC/IF, ELISA 등 다양한 응용에 적합하며, 신경줄기세포 및 종양 내 혈관신생 연구에 유용합니다. -20°C 보관, Affinity 정제 제품입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1392–1621 of human NES (NP_006608.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.49 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804789 |
Product Specific Information
- Positive Samples: LO2, 293T, Mouse brain
- Immunogen sequence:
QVPQLLLDPA AWDRDGESDG FADEEESGEE GEEDQEEGRE PGAGRWGPGS SVGSLQALSS SQRGEFLESD SVSVSVPWDD SLRGAVAGAP KTALETESQD SAEPSGSEEE SDPVSLERED KVPGPLEIPS GMEDAGPGAD IIGVNGQGPN LEGKSQHVNG GVMNGLEQSE EVGQGMPLVS EGDRGSPFQE EEGSALKTSW AGAPVHLGQG QFLKFTQREG DRESWSSGED
Target Information
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells.
It is widely used as a marker for stem/progenitor cells, glioma cells, and tumor endothelial cells.
Nestin serves as an angiogenic marker to evaluate neovascularity in tumors.
Highly specific antibodies to human Nestin aid in characterizing progenitor cells, enhancing therapeutic potential of neural stem/progenitor cells for CNS diseases or injury, and identifying neuroepithelial tumor cells.
Nestin expression is also observed in pancreatic hematopoietic stem cell populations and is considered a novel angiogenesis marker in proliferating endothelial cells of colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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