
Thermo Fisher Scientific Bub3 Recombinant Rabbit Monoclonal Antibody (JG38-81)
Bub3 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC, Flow Cytometry에 적합합니다. HEK293 세포에서 발현되었으며, Protein A로 정제된 고순도 항체입니다. 다양한 인체 및 동물 조직 샘플에서 검증되었습니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:2,000 |
| Flow Cytometry (Flow) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JG38-81 |
| Immunogen | Synthetic peptide within Human Bub3 aa 2–50 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles, store in dark |
| Shipping Conditions | Wet ice |
| RRID | AB_2848700 |
Product Specific Information
Positive Control:
A549, HL-60, A431, rat colon tissue lysate, LOVO, rat testis tissue, human thyroid gland tissue, human colon cancer tissue, mouse fallopian tissue, PC-3M.
Target Information
This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and shows sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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