Thermo Fisher Scientific Lamin B2 Recombinant Rabbit Monoclonal Antibody (ARC1252)
Lamin B2 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF, ELISA에 사용 가능. 인간, 생쥐, 랫트 반응성. HEK293 발현 시스템에서 생산되며, 고순도의 Affinity Chromatography 정제. -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC1252 |
| Immunogen | Synthetic peptide corresponding to amino acids 521–620 of human Lamin B2 (UniProt ID: Q03252) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849518 |
Product Specific Information
Immunogen sequence:
YILRAGQMVT VWAAGAGVAH SPPSTLVWKG QSSWGTGESF RTVLVNADGE EVAMRTVKKS SVMRENENGE EEEEEAEFGE EDLFHQQGDP RTTSRGCYVM
Target Information
Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic side of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished: A-type and B-type lamins.
- A-type lamins: Lamin A, Lamin C, Lamin Adel 10 (from the same gene by alternative splicing)
- B-type lamins: Lamin B1 and Lamin B2 (from two distinct genes)
Mutations in A-type lamins are associated with rare dominant genetic disorders such as:
- Emery-Dreifuss muscular dystrophy
- Dilated cardiomyopathy with conduction-system disease
- Dunnigan-type familial partial lipodystrophy
A-type lamins are expressed during cell differentiation and interact with chromatin, suggesting a role in differential gene expression regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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