Thermo Fisher Scientific PKC epsilon Recombinant Rabbit Monoclonal Antibody (ARC1270)
PKC epsilon 단백질을 인식하는 토끼 단클론 항체로, Western blot과 ELISA에 적합합니다. HEK293 세포에서 발현된 재조합 항체이며, 높은 특이성과 재현성을 제공합니다. 연구용으로 세포 신호전달 및 PKC 관련 연구에 활용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC1270 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 500–600 of human PKC epsilon (Q02156) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.94 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849517 |
Product Specific Information
Immunogen sequence:
RSRKFDEPRS RFYAAEVTSA LMFLHQHGVI YRDLKLDNIL LDAEGHCKLA DFGMCKEGIL NGVTTTTFCG TPDYIAPEIL QELEYGPSVD WWALGVLMYE M
Target Information
The PKC family of serine/threonine kinases, including PRKCE (PKC epsilon), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity.
PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca²⁺- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. They also serve as major receptors for phorbol esters, a class of tumor promoters. Each PKC family member has a specific expression profile and distinct cellular role.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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