Thermo Fisher Scientific MCHR2 Polyclonal Antibody
Thermo Fisher Scientific의 MCHR2 Polyclonal Antibody는 사람 MCHR2 단백질을 인식하는 토끼 다클론 항체로, IHC(P) 실험에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며 PBS 버퍼에 0.1% sodium azide가 포함되어 있습니다. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic 18 amino acid peptide from C-terminal cytoplasmic domain of human MCHR2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2550379 |
Product Specific Information
- Percent identity with other species by BLAST analysis:
Human (100%), Gorilla, Gibbon (94%), Monkey, Marmoset (89%), Panda, Pig (83%) - For IHC(P): Use heat-induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Target Information
Melanin-concentrating hormone (MCH) is a 19 amino acid cyclic neuropeptide mainly expressed in the hypothalamus. MCH modulates feeding behavior, aggression, anxiety, arousal, and reproductive function in mammals by controlling the release of luteinizing hormone (LH).
The melanin-concentrating hormone receptor (MCHR, also designated SLC-1) is a glycosylated G-protein coupled receptor that migrates between 45–50 kDa on SDS-PAGE. MCHR mediates the effects of MCH through Gai and/or Gaq signaling and is expressed in several regions of the brain, including the cerebral cortex, amygdala, thalamus, and hypothalamus.
MCH and MCHR have also been implicated in stimulating leptin expression and secretion in adipocytes, suggesting that the melanin-concentrating hormone and its receptor may be potential targets for modulating obesity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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