Thermo Fisher Scientific MTDH Monoclonal Antibody (OTI6H2), TrueMAB
MTDH 단백질을 인식하는 Mouse IgG2a 단일클론 항체로, Western blot 및 IHC(P) 분석에 적합합니다. 인간 Metadherin(AA 70-582) 재조합 단백질로부터 제작되었으며, 무보존제 PBS 버퍼에 동결건조 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI6H2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 70–582 of human MTDH produced in SF9 cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Metadherin (MTDH), also known as LYRIC, is a novel protein associated with tight junction proteins ZO-1 and occludin in polarized epithelial cells.
It is not a structural component but is recruited during tight junction maturation.
MTDH is overexpressed in breast cancer tissues and xenografts, while expression is lower in normal breast tissue.
It binds to lung vasculature via its extracellular C-terminal domain; blocking this domain or inhibiting MTDH with siRNA has been shown to reduce breast cancer metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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