Thermo Fisher Scientific FceR1 alpha Monoclonal Antibody (MAR-1), PE-eFluor 610, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

Description:
The MAR-1 monoclonal antibody reacts with the Fc epsilon Receptor I alpha subunit (FcεRIα), an IgE-binding subunit lacking signal-transducing ability. FcεRIα is expressed on mast and basophil cells and is up-regulated by the presence of IgE. It forms a tetrameric complex with one beta and two gamma subunits containing immunoreceptor tyrosine-based activation motifs (ITAMs). The FcεRI complex is key in triggering IgE-mediated allergic reactions.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Tested in flow cytometric analysis of mouse bone marrow-derived mast cells. Recommended ≤0.25 µg per test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Optimize titration for best performance.

Fluorochrome Characteristics:
PE-eFluor® 610 is excited by 488–561 nm laser lines and emits at 607 nm. Recommended detection using a 610/20 band pass filter (equivalent to PE-Texas Red®). Ensure instrument compatibility.

Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product #00-8222)
• 1-step Fix/Lyse Solution (Product #00-5333)
  Clone-specific performance should be verified empirically.

Excitation/Emission: 488–561 nm / 607 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

FceR1 (Fc epsilonR1 alpha) is a subunit of the high-affinity IgE receptor. It forms a tetrameric complex (αβγ₂), where the beta and gamma subunits mediate signal transduction. FcεRI plays a central role in allergic responses by coupling allergen-bound IgE with mast cells, leading to histamine and mediator release. This process initiates inflammatory responses characteristic of allergic disorders such as hay fever and asthma.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

제품 이미지