Thermo Fisher Scientific H2BK5ac Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Immunocytochemistry (ICC/IF)

1:100

-

Immunoprecipitation (IP)

1:50

-

ChIP assay (ChIP)

1.5 µg/1x10^6 cells

-

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic acetylated peptide corresponding to residues surrounding Lys5 of human histone H2B if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:H2BK5ac,uniProtId:P33778-1,ncbiNodeId:9606,antigenRange:5,antigenLength:126,antigenImageFileName:PA5-17779_H2BK5ac_P33778-1_Rabbit.svg,antigenImageFileNamePDP:PA5-17779_H2BK5ac_P33778-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:H2BK5ac,uniProtId:Q93079-1,ncbiNodeId:9606,antigenRange:5,antigenLength:126,antigenImageFileName:PA5-17779_H2BK5ac_Q93079-1_Rabbit.svg,antigenImageFileNamePDP:PA5-17779_H2BK5ac_Q93079-1_Rabbit_PDP.jpeg,sortOrder:10},{targetFamily:H2BK5ac,uniProtId:Q99877-1,ncbiNodeId:9606,antigenRange:5,antigenLength:126,antigenImageFileName:PA5-17779_H2BK5ac_Q99877-1_Rabbit.svg,antigenImageFileNamePDP:PA5-17779_H2BK5ac_Q99877-1_Rabbit_PDP.jpeg,sortOrder:11},{targetFamily:H2BK5ac,uniProtId:Q99879-1,ncbiNodeId:9606,antigenRange:5,antigenLength:126,antigenImageFileName:PA5-17779_H2BK5ac_Q99879-1_Rabbit.svg,antigenImageFileNamePDP:PA5-17779_H2BK5ac_Q99879-1_Rabbit_PDP.jpeg,sortOrder:12},{targetFamily:H2BK5ac,uniProtId:Q99880-1,ncbiNodeId:9606,antigenRange:5,antigenLength:126,antigenImageFileName:PA5-17779_H2BK5ac_Q99880-1_Rabbit.svg,antigenImageFileNamePDP:PA5-17779_H2BK5ac_Q99880-1_Rabbit_PDP.jpeg,sortOrder:13},{targetFamily:H2BK5ac,uniProtId:P57053-1,ncbiNodeId:9606,antigenRange:5,antigenLength:126,antigenImageFileName:PA5-17779_H2BK5ac_P57053-1_Rabbit.svg,antigenImageFileNamePDP:PA5-17779_H2BK5ac_P57053-1_Rabbit_PDP.jpeg,sortOrder:2},{targetFamily:H2BK5ac,uniProtId:P62807-1,ncbiNodeId:9606,antigenRange:5,antigenLength:126,antigenImageFileName:PA5-17779_H2BK5ac_P62807-1_Rabbit.svg,antigenImageFileNamePDP:PA5-17779_H2BK5ac_P62807-1_Rabbit_PDP.jpeg,sortOrder:3},{targetFamily:H2BK5ac,uniProtId:O60814-1,ncbiNodeId:9606,antigenRange:5,antigenLength:126,antigenImageFileName:PA5-17779_H2BK5ac_O60814-1_Rabbit.svg,antigenImageFileNamePDP:PA5-17779_H2BK5ac_O60814-1_Rabbit_PDP.jpeg,sortOrder:4},{targetFamily:H2BK5ac,uniProtId:P06899-1,ncbiNodeId:9606,antigenRange:5,antigenLength:126,antigenImageFileName:PA5-17779_H2BK5ac_P06899-1_Rabbit.svg,antigenImageFileNamePDP:PA5-17779_H2BK5ac_P06899-1_Rabbit_PDP.jpeg,sortOrder:5},{targetFamily:H2BK5ac,uniProtId:P23527-1,ncbiNodeId:9606,antigenRange:5,antigenLength:126,antigenImageFileName:PA5-17779_H2BK5ac_P23527-1_Rabbit.svg,antigenImageFileNamePDP:PA5-17779_H2BK5ac_P23527-1_Rabbit_PDP.jpeg,sortOrder:6},{targetFamily:H2BK5ac,uniProtId:Q16778-1,ncbiNodeId:9606,antigenRange:5,antigenLength:126,antigenImageFileName:PA5-17779_H2BK5ac_Q16778-1_Rabbit.svg,antigenImageFileNamePDP:PA5-17779_H2BK5ac_Q16778-1_Rabbit_PDP.jpeg,sortOrder:7},{targetFamily:H2BK5ac,uniProtId:Q5QNW6-1,ncbiNodeId:9606,antigenRange:5,antigenLength:126,antigenImageFileName:PA5-17779_H2BK5ac_Q5QNW6-1_Rabbit.svg,antigenImageFileNamePDP:PA5-17779_H2BK5ac_Q5QNW6-1_Rabbit_PDP.jpeg,sortOrder:8},{targetFamily:H2BK5ac,uniProtId:Q8N257-1,ncbiNodeId:9606,antigenRange:5,antigenLength:126,antigenImageFileName:PA5-17779_H2BK5ac_Q8N257-1_Rabbit.svg,antigenImageFileNamePDP:PA5-17779_H2BK5ac_Q8N257-1_Rabbit_PDP.jpeg,sortOrder:9}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10988439

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other acetylated histones.

Target Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The nucleosomes wrap further and compact DNA into chromotin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones play a central role in transciption regulation, DNA repair, DNA replication, and chromosomal stability. H2B has a broad antibacterial activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.