Thermo Fisher Scientific BPI Polyclonal Antibody
Thermo Fisher Scientific의 BPI Polyclonal Antibody는 인간 BPI 단백질을 인식하는 Rabbit IgG 항체로, WB, IHC, Flow Cytometry 등 다양한 연구 응용에 적합합니다. 고순도 정제 및 안정적 액상 형태로 제공되며, 항균 단백질 BPI 연구에 유용합니다.
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Thermo Fisher Scientific BPI Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 | - |
| Flow Cytometry (Flow) | 1:10–1:50 | - |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide between 293–324 amino acids from the central region of human BPI |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Ammonium sulfate precipitation, Size-exclusion, Dialysis |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2543569 |
Target Information
The antimicrobial protein BPI is a 55 kDa protein found in the primary (azurophilic) granules of human neutrophils and also on the surface of neutrophils, small intestinal, and oral epithelial cells.
BPI is a bactericidal compound present in polymorphonuclear cells (PMN) and eosinophils. It binds strongly to the lipid A region of lipopolysaccharides (LPS) in gram-negative bacteria, leading to multiple anti-infective activities such as:
- Cytotoxicity through damage to bacterial membranes
- Neutralization of bacterial LPS
- Opsonization to enhance phagocytosis by neutrophils
BPI may play a key role in LPS-triggered airway disease, as airway epithelial cells express and produce BPI. Inflammation induced by LPS can contribute to rapid airflow decline, a severe complication of hematopoietic cell transplantation.
Additionally, a 21 kDa recombinant fragment, rBPI21, has shown protective effects against invasive pneumococcal disease by binding to pneumolysin, a gram-positive bacterial toxin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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