Thermo Fisher Scientific LIN28A Monoclonal Antibody (14E6-4E6)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

View 1 publication 1 publication

Flow Cytometry (Flow)

1:100

-

Immunoprecipitation (IP)

5 µg

-

ChIP assay (ChIP)

1-3 µL

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

14E6-4E6

Immunogen

Full-length human recombinant protein expressed in bacteria if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:LIN28A,uniProtId:Q9H9Z2-1,ncbiNodeId:9606,antigenRange:1-209,antigenLength:209,antigenImageFileName:MA1-016_LIN28A_Q9H9Z2-1_House_mouse.svg,antigenImageFileNamePDP:MA1-016_LIN28A_Q9H9Z2-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• DyLight 488
• DyLight 650
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 30% glycerol, 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536672

Product Specific Information

MA1-016 has been successfully used in Western blot, immunofluorescence, ChIP, flow cytometry, IHC (P) and immunoprecipitation applications on human and mouse samples.

Target Information

Lin28 is a transcription factor that was first identified through its key role in the pathway of developmental timing in C. elegans. The role of Lin28 in development suggested that it might be useful in the creation of stem cells that might be beneficial in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing Lin28 in addition to the transcription factors POU5F1, Sox2, and NANOG in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing Lin28, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.