Thermo Fisher Scientific LIN28A Monoclonal Antibody (14E6-4E6)
LIN28A 단백질을 인식하는 마우스 모노클로날 항체로, Western blot, IHC, ICC, Flow cytometry, ChIP 등 다양한 응용에 적합합니다. 인간 및 생쥐 시료에서 검증되었으며, 단백질 A로 정제된 액상 형태로 제공됩니다. 줄기세포 연구 및 발달 생물학 연구에 활용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | View 1 publication |
| Flow Cytometry (Flow) | 1:100 | - |
| Immunoprecipitation (IP) | 5 µg | - |
| ChIP assay (ChIP) | 1–3 µL | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 14E6-4E6 |
| Immunogen | Full-length human recombinant LIN28A protein (amino acids 1–209) expressed in bacteria |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 30% glycerol, 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536672 |
Additional Formats
Product Specific Information
MA1-016 has been successfully used in Western blot, immunofluorescence, ChIP, flow cytometry, IHC (P), and immunoprecipitation applications on human and mouse samples.
Target Information
Lin28 is a transcription factor first identified for its role in developmental timing in C. elegans. It plays a key role in stem cell biology and is used in generating induced pluripotent stem (iPS) cells when co-expressed with transcription factors such as POU5F1, Sox2, and NANOG. iPS cells can also be generated using plasmids expressing Lin28, Sox2, POU5F1, and c-Myc, eliminating the need for viral introduction and improving safety for regenerative medicine applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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