Thermo Fisher Scientific LIN28A Monoclonal Antibody (14E6-4E6), DyLight 650

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Flow Cytometry (Flow)

1:50-1:500

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Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

14E6-4E6

Immunogen

Full-length human recombinant protein expressed in bacteria if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:LIN28A,uniProtId:Q9H9Z2-1,ncbiNodeId:9606,antigenRange:1-209,antigenLength:209,antigenImageFileName:MA1-016-D650_LIN28A_Q9H9Z2-1_House_mouse.svg,antigenImageFileNamePDP:MA1-016-D650_LIN28A_Q9H9Z2-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

DyLight™ 650 DyLight™ 650 DyLight™ 650

View additional formats

• Unconjugated
• DyLight 488
• Request custom conjugation

Excitation/Emission Max

651/673 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with proprietary stabilizer

Contains

0.02% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536675

Product Specific Information

MA1-016-D650 has been successfully used in ICC/IF and flow cytometry applications on human and mouse samples.

MA1-016-D650 can be used for immunofluorescence analysis of LIN28 in human iPSCs.

Target Information

Lin28 is a transcription factor that was first identified through its key role in the pathway of developmental timing in C. elegans. The role of Lin28 in development suggested that it might be useful in the creation of stem cells that might be beneficial in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing Lin28 in addition to the transcription factors POU5F1, Sox2, and NANOG in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing Lin28, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.