Thermo Fisher Scientific GLI1 Monoclonal Antibody (OTI4C1), TrueMAB
GLI1 단백질을 인식하는 Mouse IgG1 단일클론 항체로 Western blot 및 IHC(P) 실험에 적합. Human에 반응하며, 무보존제 PBS buffer에 동결건조 형태로 제공. 재구성 및 결합 실험에 최적화된 고순도 항체.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4C1 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 755–1106 of human GLI1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
GLI1 encodes a member of the Kruppel family of zinc finger proteins. This transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. Its activity and nuclear localization are negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been identified for this gene.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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