Thermo Fisher Scientific RPN1 Monoclonal Antibody (OTI3E1), TrueMAB
RPN1 단백질을 표적하는 Mouse IgG1 단일클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry에 적합합니다. 인간 및 랫트 반응성을 가지며, 동결건조 형태로 제공되어 재구성 후 1 mg/mL 농도로 사용 가능합니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific RPN1 Monoclonal Antibody (OTI3E1), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3E1 |
| Immunogen | Full length human recombinant protein of human RPN1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Ribophorins 1 and 2 are abundant and highly conserved glycoproteins located in the endoplasmic reticulum, participating in ribosome binding.
Mammalian oligosaccharyltransferase activity is associated with a complex composed of RPN1, RPN2, and oligosaccharyltransferase proteins.
RPN1 is a component of the proteasome base, and the ubiquitin-like (UBL) domain of recombinant Rad23 interacts with proteasomes through the leucine-rich repeat domain of RPN1.
The RPN1 gene maps to chromosome 3, and RPN2 maps to chromosome 20 by in situ hybridization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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