
Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor Plus 488
Alexa Fluor Plus 488 형광 표지를 가진 염소 유래 항-마우스 IgG(H+L) 2차 항체로, 높은 특이성과 로트 간 일관성을 제공하는 재조합 Superclonal 항체입니다. Western blot, ICC/IF, Flow cytometry 등에 최적화되어 있으며, 밝은 형광 신호와 낮은 배경으로 정밀한 이미징이 가능합니다.
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Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor Plus 488
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–4 µg/mL |
| Flow Cytometry (Flow) | 1–4 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Goat / IgG |
| Expression System | Expi293 |
| Class | Recombinant Superclonal |
| Type | Secondary Antibody |
| Immunogen | Purified Mouse Immunoglobulin |
| Conjugate | Alexa Fluor™ Plus 488 |
| Excitation/Emission Max | 493/518 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 6.5 |
| Contains | 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2921066 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro, providing improved specificity and superior lot-to-lot consistency.
Minimal cross-reactivity with rabbit, rat, human, bovine, guinea pig, and donkey IgG is observed.
Alexa Fluor Plus secondary antibodies deliver brighter fluorescence signals compared to conventional Alexa Fluor conjugates, enhancing signal-to-noise ratios for critical imaging experiments.
These antibodies utilize proprietary dye chemistry for superior visualization of low-abundance targets and reduced optimization time.
Usage Tip:
Before use, centrifuge the conjugate solution briefly and use only the supernatant to remove possible aggregates and reduce nonspecific background staining. Optimal dilution should be determined empirically for each application.
Target Information
Anti-Mouse secondary antibodies are affinity-purified reagents with well-characterized specificity for mouse immunoglobulins. They are widely used for detection, sorting, or purification of mouse IgG targets.
Secondary antibodies amplify signals by binding multiple molecules to a single primary antibody, increasing sensitivity across fluorescence or enzymatic detection systems.
They are typically produced by immunizing host animals with pooled mouse immunoglobulins and further purified by immunoaffinity chromatography or conjugation methods.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Available Conjugate Formats
- Alexa Fluor Plus 405
- Alexa Fluor Plus 647
- Alexa Fluor 555
- Alexa Fluor 647
- Alexa Fluor 680
- Alexa Fluor 790
- DyLight 350, 405, 488, 550, 594, 633, 650, 680, 800 4X PEG
- FITC, TRITC, Rhodamine, PE, HRP, AP, Biotin, Agarose, WesternDot 625
- Custom conjugation available upon request
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