Thermo Fisher Scientific Goat anti-Human IgG Fc Recombinant Secondary Antibody
Recombinant goat anti-human IgG Fc secondary antibody for WB, ICC, ELISA, and peptide array. High specificity for human IgG Fc without cross-reactivity. Recombinant production ensures lot-to-lot consistency and animal origin-free formulation. Suitable ...
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Thermo Fisher Scientific Goat anti-Human IgG Fc Recombinant Secondary Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:1,000 |
| ELISA | 1:1,000–1:2,000 |
| Peptide Array | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Secondary Antibody |
| Clone | 3H8L9 |
| Immunogen | Purified Human IgG |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C and avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2925760 |
| Target | IgG Fc |
| Antibody Form | Whole Antibody |
Product Specific Information
This Goat anti-Human IgG Fc Recombinant Secondary Antibody specifically detects human IgG Fc with no cross-reactivity to human IgA, IgM, or immunoglobulins from other species.
Each lot’s sensitivity and specificity are confirmed using ELISA.
Recombinant antibodies are produced using cloned genes encoding the desired antibody, expressed in vitro.
Advantages include:
- Enhanced specificity
- Animal origin-free formulation
- Lot-to-lot consistency
It is recommended to titrate the antibody for optimal assay performance.
Target Information
Anti-Human secondary antibodies are affinity-purified and exhibit well-characterized specificity for human immunoglobulins.
They are useful in detection, sorting, or purification of target proteins.
Secondary antibodies provide versatility for various detection systems (HRP, AP, fluorescence) and improved sensitivity via signal amplification.
Typically, secondary antibodies are generated by immunizing host animals with pooled immunoglobulins from the target species, followed by purification and modification (e.g., immunoaffinity chromatography, fragmentation, conjugation) to yield highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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