
Thermo Fisher Scientific Goat anti-Human IgG1 (Heavy chain) Recombinant Secondary Antibody
인간 IgG1 중쇄에 특이적인 염소 유래 재조합 단클론 2차 항체. 높은 특이성과 감도를 제공하며, 다양한 면역분석(WB, ICC, ELISA 등)에 사용 가능. 동물 유래 성분이 없는 일관된 로트 간 품질 유지. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:1,000 |
| ELISA | 1:1,000 |
| Peptide Array | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Secondary Antibody |
| Clone | 3H60L9 |
| Immunogen | Purified Human IgG |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2925758 |
| Target | IgG1 (Heavy chain) |
| Antibody Form | Whole Antibody |
Product Specific Information
This Goat anti-Human IgG1 (Heavy chain) Recombinant Monoclonal Antibody reacts specifically with the human IgG1 heavy chain without cross-reactivity to IgG2, IgG3, IgG4, IgA, IgM, or other species immunoglobulins (including monkey IgG).
Each lot’s sensitivity and specificity are confirmed using ELISA.
Recombinant antibodies are generated by cloning specific genes encoding the desired antibody into an expression vector and producing them in vitro.
Advantages include:
- Improved specificity
- Animal origin-free formulation
- Lot-to-lot consistency
It is recommended to titrate the antibody for optimal performance in the desired assay.
Target Information
Anti-Human secondary antibodies are affinity-purified with well-defined specificity for human immunoglobulins. They are useful for detection, sorting, or purification of the specified target.
Secondary antibodies enhance versatility and sensitivity by enabling multiple detection systems (HRP, AP, fluorescence) and signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, these antibodies are produced by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to yield highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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