Thermo Fisher Scientific LIF Polyclonal Antibody
Human LIF 단백질을 인식하는 Goat Polyclonal Antibody로, IHC(P) 및 Neutralization 실험에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공됩니다. 인간 시료에 반응하며, 교차반응은 30% 미만입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–15 µg/mL |
| Neutralization (Neu) | 0.04–0.08 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived recombinant human LIF (Ser23–Phe202) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609406 |
Product Specific Information
- In direct ELISAs and Western blots, less than 30% cross-reactivity with recombinant mouse LIF is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Endotoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
Leukemia inhibitory factor (LIF) is a 20 kDa protein belonging to the IL-6 receptor family. It binds to a heterodimeric receptor composed of a LIF-specific subunit (gp190 or LIFR) and gp130, which is shared with other IL-6 family members. LIF expression occurs in various tissues including thymus, lung, and neuronal tissue. It can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels have been observed in rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF is known for inhibiting differentiation of embryonic stem cells in mice and supporting stem cell self-renewal. Human and mouse LIF share 79% sequence homology, but the inhibition of stem cell differentiation is mouse-specific.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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