Thermo Fisher Scientific Plasma Kallikrein Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

-

Immunoprecipitation (IP)

25 µg/mL

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Kallikrein/KLKB1 Gly20-Ala638 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Plasma Kallikrein,uniProtId:P26262-1,ncbiNodeId:10090,antigenRange:20-638,antigenLength:638,antigenImageFileName:PA5-47334_Plasma_Kallikrein_P26262-1_Goat.svg,antigenImageFileNamePDP:PA5-47334_Plasma_Kallikrein_P26262-1_Goat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609106

Product Specific Information

In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant human KLKB1 is observed and less than 1% cross-reactivity with recombinant human KLK1, 3, 5, 7, 13, and 14 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

Plasma prekallikrein is a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. It is synthesized in the liver and secreted into the blood as a single polypeptide chain. Plasma prekallikrein is converted to plasma kallikrein by factor XIIa by the cleavage of an internal Arg-Ile bond. Plasma kallikrein therefore is composed of a heavy chain and a light chain held together by a disulphide bond. The heavy chain originates from the amino-terminal end of the zymogen and contains 4 tandem repeats of 90 or 91 amino acids. Each repeat harbors a novel structure called the apple domain. The heavy chain is required for the surface-dependent pro-coagulant activity of plasma kallikrein. The light chain contains the active site or catalytic domain of the enzyme and is homologous to the trypsin family of serine proteases. Plasma prekallikrein deficiency causes a prolonged activated partial thromboplastin time in patients.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.