Thermo Fisher Scientific V5 Tag Polyclonal Antibody
V5 태그 단백질 검출용 Thermo Fisher Scientific의 염소 유래 폴리클로날 항체입니다. WB, ICC/IF, ELISA, IP 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제된 고순도 제품. 4°C 보관, 연구용으로만 사용.
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Thermo Fisher Scientific V5 Tag Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 - 1:30,000 |
| Immunocytochemistry (ICC/IF) | 1:100 - 1:400 |
| ELISA | 1:1,000 - 1:30,000 |
| Immunoprecipitation (IP) | 1 - 4 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide representing amino acid residues 95–108 (GKPIPNPLLGLDST) of RNA polymerase α subunit of simian virus 5 conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Goats were immunized with T7 (MASMTGGQQMG) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Target Information
Epitope tags provide a method to localize gene products in various cell types, study protein topology and complexes, identify associated proteins, and characterize newly identified or poorly immunogenic proteins when specific antibodies are not available.
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). It is typically used with all 14 amino acids (GKPIPNPLLGLDST), though shorter variants (IPNPLLGLD) are also used.
Usage Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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