Thermo Fisher Scientific V5 Tag Polyclonal Antibody, HRP
V5 태그 단백질 검출용 HRP 결합 다클론 항체. Western blot, ICC/IF, ELISA에 적합. 토끼 유래 IgG로 항원 친화 크로마토그래피 정제. 4°C 보관, 연구용 전용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 - 1:30,000 |
| Immunocytochemistry (ICC/IF) | 1:200 - 1:500 |
| ELISA | 1:10,000 - 1:100,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.05% Pro-Clean 400 |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
Rabbits were immunized with T7 (MASMTGGQQMG) conjugated to KLH.
Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Target Information
Epitope tags provide a method to localize gene products in various cell types, study protein topology and complexes, identify associated proteins, and characterize low-abundance or poorly immunogenic proteins when specific antibodies are not available.
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5).
The V5 tag typically includes all 14 amino acids (GKPIPNPLLGLDST), though a shorter 9 amino acid sequence (IPNPLLGLD) is also used.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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