Thermo Fisher Scientific MYO1A Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended positive controls: Jurkat, Raji, K562, NCI-H929
• Predicted reactivity: Mouse (93%), Rat (94%), Bovine (94%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene belongs to the myosin superfamily. Myosins are molecular motors that, upon interaction with actin filaments, utilize energy from ATP hydrolysis to generate mechanical force. Each myosin has a conserved N-terminal motor domain containing ATP-binding and actin-binding sequences. Following the motor domain is a light-chain-binding neck region with IQ motifs that bind calmodulin or other EF-hand calcium-binding proteins. The C-terminal tail domain is distinct for each myosin class and is involved in dimerization, membrane binding, protein interactions, and subcellular targeting.

In kidney epithelial cell line LLC-PK1-CL4 (CL4), the encoded protein forms a brush border on the apical surface. The brush border population of this protein turns over rapidly, while its head and tail domains interact transiently with actin and plasma membrane. A rapidly exchanging pool of this protein envelops a static actin core bundle.

Safety Information

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.