Thermo Fisher Scientific Histone H3 Polyclonal Antibody
Histone H3 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, ICC/IF, IP, ChIP 등 다양한 응용에 적합합니다. 인간, 생쥐, 랫드 시료에 반응하며, 고순도 항원 친화 크로마토그래피 정제 제품입니다. 보존제 무첨가, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:4,000 | View 16 publications |
| Immunocytochemistry (ICC/IF) | 1:250 | - |
| Immunoprecipitation (IP) | - | View 2 publications |
| ChIP assay (ChIP) | 1:50 | View 3 publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse, Xenopus, Yeast |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the carboxy-terminal sequence of human histone H3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 555 µg/mL |
| Purification | Antigen affinity chromatography, Protein A |
| Storage Buffer | 0.01M HEPES (pH 7.5), 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10985434 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with other histones.
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with other core histones, it binds DNA to form nucleosomes, the fundamental units of chromatin structure. Histone H3 plays a crucial role in transcription regulation, DNA repair, replication, and chromosomal stability.
Post-translational modifications such as methylation, acetylation, and phosphorylation of its N-terminal tail regulate chromatin structure and gene expression.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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