Thermo Fisher Scientific CD45RA Monoclonal Antibody (PTPRC, 818)
인간 CD45RA 단백질을 인식하는 Mouse IgG2a 단일클론 항체로, WB, IHC, ICC/IF, Flow Cytometry에 사용 가능. 단백질 A/G로 정제된 액상 형태이며, 4°C 보관. 면역세포 신호전달 및 발달 연구에 적합.
- 카탈로그번호
- 5788-MSM14-P1
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD45RA Monoclonal Antibody (PTPRC, 818)
Thermo Fisher Scientific CD45RA Monoclonal Antibody (PTPRC, 818)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PTPRC, 818 |
| Immunogen | Stimulated human leukocytes |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 min.
Target Information
CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes.
CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, regulating Src-family kinases.
Multiple isoforms exist due to alternative splicing; they differ in extracellular domains but share identical transmembrane and cytoplasmic domains.
CD45 isoforms vary in their membrane localization and expression depending on cell type and physiological state.
Besides immunoreceptor signaling, CD45 modulates integrin-mediated signal transduction and is involved in DNA fragmentation during apoptosis.
CD45 is expressed on human hematopoietic cells except mature red cells and is absent from differentiated non-hematopoietic tissues.
Dysfunction of CD45 is associated with severe combined immunodeficiency and Hepatitis C.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(해당 항원 구조 이미지 및 제품 관련 이미지가 여기에 위치합니다.)
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.