Thermo Fisher Scientific Adenylate Kinase 4 Recombinant Rabbit Monoclonal Antibody (014)
Recombinant rabbit monoclonal antibody recognizing human AK4/AK3L1. Validated for ELISA and immunoprecipitation. Offers high specificity, consistency, and preservative-free formulation. Suitable for mitochondrial enzyme research applications.
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Applications
ELISA (ELISA)
- Tested Dilution: 1:5,000–1:10,000
Immunoprecipitation (IP)
- Tested Dilution: 1–4 µL/mg of lysate
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 014 |
| Immunogen | Recombinant Human AK4/AK3L1 protein (Ala2–Tyr223) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C (short term); -20°C (long term). Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2784958 |
Product Specific Information
This antibody is preservative-free. To prevent contamination, it is recommended to add sodium azide (final concentration 0.05%–0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
This antibody shows specificity for Human AK4/AK3L1.
Target Information
The Adenylate Kinase 4 (AK4) gene encodes a mitochondrial matrix enzyme belonging to the adenylate kinase family. These enzymes regulate the adenine and guanine nucleotide balance in cells by catalyzing phosphate group transfer among nucleotides.
There are five known vertebrate isozymes, each with tissue-specific and developmentally regulated expression.
A pseudogene is located on chromosome 17, and three transcript variants encoding the same protein have been identified.
Sequence alignment indicates the gene (NM_013410, NM_203464, NM_001005353) is located on chromosome 1.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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